Butterfly guru Nigel Venters has provided this excellend illustration which shows clearly how to tell the sex of Monarch butterfly pupae.
SEXING MONARCH PUPAE can easily be done with a good magnifying lens. this image has been enhanced to show the differences you need to look for. The enlargements of the anal segments to the right of the picture give a closer view of the difference.
THE MALE PUPA is at the top of the picture, it has a small dimple on the dividing edge between the anal segment and the second segment.
THE FEMALE PUPA has a distinct indented line that extends from the middle of the anal segment to the third segment. The line divides the second segment completely.